Fungi is high in diversity and plays an important role in different bioprocesses such as saprophytism, parasitism, and symbiosis. However, little is known of the fungi resources in the Jhoushui area of Yushan National Park, therefore a survey of the advanced fungi in the area was conducted. After surveying the area a number of times, a total of 147 species specimens were collected, of which 64 were newly recorded species in Taiwan. The records indicated that the area had an abundant fungi resource. Understanding the fungi in the area would help understanding fungi’s ecological role and relationship with other organisms. Many fungi species also have nutritious and medicinal values, therefore preliminary understanding of fungi must be acquired in order to conduct in-depth studies. The information acquired through this survey will be provided for the Yushan National Park Headquarters as educational materials and exhibit illustrations.