In order to adapt to different environments, organisms response in form, physiology and behavior, which produces variations in life history. Formosan Sambar (Rusa unicolor swinhoii) has great adaptability and is widely distributed from low to high altitudes in Taiwan. Differences in vegetation, temperature, and solar radiation along the elevations may lead to variations in life history of Sambar living in different regions. This study uses camera traps to observe life history traits of Sambar in Sinkang Mountain and Shishuishan at high altitudes, Nianxi Forest Road at middle altitudes, and Zhiben Forest Road and Jiufen Ershan at low altitudes. Life history traits included antler cycle of male, fighting, grouping of male and female, mating and fawn. Timing of antler cycle differed among areas, but the difference was not correlated with altitude. Grouping of male and female and fighting occurred during the same period of time. In addition, fawn appeared later at lower altitudes. Fawn appeared earliest in Shishuishan at high altitude and latest in Zhibenlin road at low altitude. Further study on the effects of vegetation, phenology and food quality on sambar in necessary to answer why life history traits vary spatially.