Study on the diet of animals and local food sources are important for understanding how animals utilize the resources in an area to meet their energy and nutritional requirement. Different insectivorous bats may utilize different types of insects due to species, sex, and age. In addition, insectivorous bats use echolocation to detect, classify, and identify preys and obstacles during nighttime flight. Ultrasounds emitted for echolocation by different species of bats also vary depending on their diet and foraging strategies. There are at least 17 insectivorous bat species of 3 families found at Yu-Shan National Park in the reports of pass few year studies, but the information of their foraging ecology is still limited and unclear. Therefore, the purpose of this study are: 1) to investigate the species composition and dynamic of insectivorous bats and insects during the study period; 2) to study the diet of dominant insectivorous bats and the relationship between bats’ diet and insect fauna according to seasonal variation; 3) to establish the echolocation reference calls of insectivorous bats at Yu-Shan National Park and to find the correlation of bats’ echolocation with their diet. Such information will enhance our knowledge of the ecological roles of insectivorous bats at Yu-Shan National Park. We captured one re-captured Myotis latirostris, and his body weight was heavier than 2007. And the highly active time of bat and the most bat diversity were during PM6:00～ 8:00 period based on the results of bats caught by net and echolocation data. Otherwise, Myotis latirostris（55.30%） and Myotis sp.2 (15.91%) were the dominant bat species, and the Diptera and Leipidoptera were the dominant insect species in investigated area. Moreover, we found ecological niche of dominant bats were overlap on Diptera, Coleoptera, and Araneida. The detail resources use by those two sympatric and relative species is interested issue to be study in the future.